Logo do repositório
 
Publicação

Polyhydroxyalkanoate Production from Eucalyptus Bark’s Enzymatic Hydrolysate

2024-04Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorRodrigues, Thomas
dc.contributor.authorTorres, Cristiana A.V.
dc.contributor.authorMarques, Susana
dc.contributor.authorGírio, Francisco
dc.contributor.authorFreitas, Filomena
dc.contributor.authorReis, Maria A.M.
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblMolecular Diversity Preservation International (MDPI)
dc.date.accessioned2025-02-14T21:19:31Z
dc.date.available2025-02-14T21:19:31Z
dc.date.issued2024-04
dc.descriptionFunding Information: This work was financed by national funds from FCT/MCTES\u2014Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia, I.P., Minist\u00E9rio da Ci\u00EAncia, Tecnologia e Ensino Superior\u2014in the scope of the integrated project be@t (Textile Bioeconomy), which has received funding from the Environmental Fund through Component 12, Promotion of Sustainable Bioeconomy (Investment TC-C12-i01\u2014Sustainable Bioeconomy No. 02/C12-i01/202), of European funds allocated to Portugal by the Recovery and Resilience Plan (RRP) within the scope of the European Union\u2019s (EU\u2019s) Recovery and Resilience Mechanism, framed in the context of the Next-Generation EU for the period 2021\u20132026. Publisher Copyright: © 2024 by the authors.
dc.description.abstractIn recent years, polyhydroxyalkanoates (PHAs) have gained notoriety because of their desirable properties that include proven biodegradability, biocompatibility, and thermal stability, which make them suitable alternatives to fossil-based polymers. However, the widespread use of PHAs is still challenging because of their production costs, which are greatly associated with the cultivation medium used for bacterial cultivation. In Portugal, one-quarter of the forest area is covered by Eucalyptus globulus wood, making its residues a cheap, abundant, and sustainable potential carbon source for biotechnological uses. In this work, eucalyptus bark was used as the sole feedstock for PHA production in a circular bioeconomic approach. Eucalyptus bark hydrolysate was obtained after enzymatic saccharification using Cellic® CTec3, resulting in a sugar-rich solution containing glucose and xylose. Although with differing performances, several bacteria were able to grow and produce PHA with distinct compositions, using the enzymatic hydrolysate as the sole carbon source. Pseudomonas citronellolis NRRL B-2504 achieved a high cellular growth rate in bioreactor assays (24.4 ± 0.15 g/L) but presented a low accumulation of a medium-chain-length PHA (mcl-PHA) comprising the monomers hydroxydecanoate (HD, 65%), hydroxydodecanoate (HDd, 25%), and hydroxytetradecanoate (HTd, 14%). Burkholderia thailandensis E264, on the other hand, reached a lower cellular growth rate (8.87 ± 0.34 g/L) but showed a higher biopolymer accumulation, with a polyhydroxybutyrate (PHB) content in the cells of 12.3 wt.%. The new isolate, Pseudomonas sp., revealed that under nitrogen availability, it was able to reach a higher accumulation of the homopolymer PHB (31 wt.%). These results, although preliminary, demonstrate the suitability of eucalyptus bark’s enzymatic hydrolysate as a feedstock for PHA production, thus offering an exciting avenue for achieving sustainable and environmentally responsible plastic products from an undervalued forestry waste.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1860875
dc.identifier.doi10.3390/ma17081773
dc.identifier.issn1996-1944
dc.identifier.otherPURE: 106546037
dc.identifier.otherPURE UUID: 8bec14d0-e1d1-49b3-8b79-6d18b5c96eab
dc.identifier.otherScopus: 85191499985
dc.identifier.otherORCID: /0000-0002-9430-4640/work/178129297
dc.identifier.urihttp://hdl.handle.net/10362/179062
dc.identifier.urlhttps://www.scopus.com/pages/publications/85191499985
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F50006%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT//2023.03035.BD/PT
dc.subjectenzyme
dc.subjecthydrolysate
dc.subjectlignocellulose
dc.subjectpolyhydroxyalkanoates
dc.subjectsaccharification
dc.subjectGeneral Materials Science
dc.subjectCondensed Matter Physics
dc.titlePolyhydroxyalkanoate Production from Eucalyptus Bark’s Enzymatic Hydrolysateen
dc.typejournal article
degois.publication.issue8
degois.publication.titleMaterials
degois.publication.volume17
dspace.entity.typePublication
oaire.awardNumberUIDP/04378/2020
oaire.awardNumberUIDB/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardNumberUIDB/50006/2020
oaire.awardNumberUIDP/50006/2020
oaire.awardNumber2023.03035.BD
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2023.03035.BD/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication38373452-5c9c-4724-8def-f03314ecce0e
relation.isProjectOfPublicatione07cf232-4705-4b5b-b2c4-af8f25311076
relation.isProjectOfPublicationecd6da06-821d-4fff-933b-319b334dc19a
relation.isProjectOfPublicationadc84c24-ba1d-4bcd-b753-2128ce9a5faa
relation.isProjectOfPublicationae403dd6-3940-45dd-a7c4-04c6bec15552
relation.isProjectOfPublication450ac064-a9d5-4d76-b464-2c195f38502c
relation.isProjectOfPublication.latestForDiscovery450ac064-a9d5-4d76-b464-2c195f38502c

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
materials-17-01773.pdf
Tamanho:
1.77 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

Home collection (FCT)