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Molecular Beacon for Detection miRNA-21 as a Biomarker of Lung Cancer

2022-03-19Artigo científico Acesso aberto
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dc.contributor.authorAlexandre, Daniela
dc.contributor.authorTeixeira, Bernardo
dc.contributor.authorRico, André
dc.contributor.authorValente, Salete
dc.contributor.authorCraveiro, Ana
dc.contributor.authorBaptista, Pedro V.
dc.contributor.authorCruz, Carla
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-08-02T22:24:38Z
dc.date.available2022-08-02T22:24:38Z
dc.date.issued2022-03-19
dc.descriptionUID/Multi/04349/ 2019 POCI-01-0145-FEDER-022122 ROTEIRO/0031/2013-PINFRA/22161/2016 LA/P/0140/2020
dc.description.abstractLung cancer (LC) is the leading cause of cancer-related death worldwide. Although the diagnosis and treatment of non-small cell lung cancer (NSCLC), which accounts for approximately 80% of LC cases, have greatly improved in the past decade, there is still an urgent need to find more sensitive and specific screening methods. Recently, new molecular biomarkers are emerging as potential non-invasive diagnostic agents to screen NSCLC, including multiple microRNAs (miRNAs) that show an unusual expression profile. Moreover, peripheral blood mononuclear cells’ (PBMCs) miRNA profile could be linked with NSCLC and used for diagnosis. We developed a molecular beacon (MB)-based miRNA detection strategy for NSCLC. Following PBMCs isolation and screening of the expression profile of a panel of miRNA by RT-qPCR, we designed a MB targeting of up-regulated miR-21-5p. This MB 21-5p was characterized by FRET-melting, CD, NMR and native PAGE, allowing the optimization of an in-situ approach involving miR-21-5p detection in PBMCs via MB. Data show the developed MB approach potential for miR-21-5p detection in PBMCs from clinical samples towards NSCLC.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent14
dc.format.extent1978274
dc.identifier.doi10.3390/ijms23063330
dc.identifier.issn1661-6596
dc.identifier.otherPURE: 43040262
dc.identifier.otherPURE UUID: d5296527-dae0-408c-98ec-0550afe91056
dc.identifier.otherScopus: 85126857201
dc.identifier.otherPubMed: 35328750
dc.identifier.otherWOS: 000775308100001
dc.identifier.otherPubMedCentral: PMC8955680
dc.identifier.otherORCID: /0000-0001-5255-7095/work/116728573
dc.identifier.urihttp://hdl.handle.net/10362/142794
dc.identifier.urlhttps://www.scopus.com/pages/publications/85126857201
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationExploring one-hit-multiple-target strategies for Lung Cancer
dc.relationinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/2021.07695.BD/PT
dc.subjectDiagnosis
dc.subjectLung cancer
dc.subjectMicroRNAs
dc.subjectMolecular beacon
dc.subjectPeripheral blood mononuclear cells
dc.subjectCatalysis
dc.subjectMolecular Biology
dc.subjectSpectroscopy
dc.subjectComputer Science Applications
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.subjectInorganic Chemistry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleMolecular Beacon for Detection miRNA-21 as a Biomarker of Lung Canceren
dc.typejournal article
degois.publication.issue6
degois.publication.titleInternational Journal of Molecular Sciences
degois.publication.volume23
dspace.entity.typePublication
oaire.awardNumberUIDP/00709/2020
oaire.awardNumberUIDB/00709/2020
oaire.awardNumberUIDP/04378/2020
oaire.awardNumberUIDB/04378/2020
oaire.awardNumber2021.07695.BD
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleExploring one-hit-multiple-target strategies for Lung Cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/2021.07695.BD/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
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