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The healing process of vertebral body fracture in Wistar rats

2025-09-30Artigo científico Acesso aberto
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dc.contributor.authorMoura, Diogo Lino
dc.contributor.authorCasal, Diogo
dc.contributor.authorCasal, Diogo
dc.contributor.authorAlves, Sara
dc.contributor.authorAlves, Sara
dc.contributor.authorAlmeida, Rui
dc.contributor.authorPais, Diogo
dc.contributor.authorPAIS, DIOGO
dc.contributor.authorCasanova, José
dc.contributor.authorBernardes, António
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblAME Publishing Company
dc.date.accessioned2025-11-18T21:10:53Z
dc.date.available2025-11-18T21:10:53Z
dc.date.issued2025-09-30
dc.descriptionPublisher Copyright: Copyright © 2025 AME Publishing Company. All rights reserved.
dc.description.abstractBackground: Although recognition of avascular necrosis of the vertebral body in post-traumatic cases has increased, it remains underdiagnosed and is one of the most unpredictable and challenging complications in spinal trauma. Vertebral arterial supply may play a key role in fracture healing, yet this remains unproven and is not currently considered in treatment algorithms. Surgical decisions between preserving or replacing the vertebral body in burst fractures are difficult, mainly due to limited knowledge of the biological factors influencing bone repair. This study aims to demonstrate the impact of vertebral vascular disruption on vertebral body fracture healing. We developed an experimental model in Wistar rats to replicate an L1 vertebral body fracture and analyze the normal healing sequence. Additionally, we examined the effects of disrupting the anterolateral blood supply on bone regeneration. Methods: Seventy female Wistar rats were divided into two groups. Group 1 (n=35) underwent an L1 burst fracture induced by ultrasonic tools. Group 2 (n=35) received the same fracture, followed by electrocauterization of the anterolateral vertebral surfaces and placement of a synthetic barrier to prevent revascularization. Vertebral specimens were collected weekly for 6 weeks. Healing was assessed macroscopically and histologically using an image-processing algorithm trained to identify inflammatory, fibroblastic/cartilaginous, and bone tissue. The predominant tissue type was used to determine the healing stage. Results: At week 1, the vascular disruption group showed significantly more inflammatory tissue (56.34%) than controls (24.25%, P=0.03), while fibroblastic/cartilaginous tissue was more common in controls (58.82% vs. 19.18%, P=0.03). By week 6, this tissue remained more prevalent in the intervention group (37.4%), while bone tissue predominated in controls (66.71% vs. 45.54%, P=0.009). Among animals already in the bone phase, trabecular structures were significantly more developed in controls (80% vs. 20%, P=0.031). Notably, all control animals reached the soft callus stage by week 1, whereas intervention animals only transitioned out of the inflammatory phase after the first week. Across all phases, progression was consistently faster in the control group, with statistical significance in the soft callus stage (P=0.002). Conclusions: Disruption of the anterolateral vascular supply significantly delays healing in L1 vertebral body fractures in rats, evidenced by slower phase transitions and reduced bone maturation. These findings underscore the essential role of vascularization in successful vertebral repair and suggest it should be considered in future therapeutic strategies.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent28
dc.format.extent3709344
dc.identifier.doi10.21037/jss-25-55
dc.identifier.issn2414-469X
dc.identifier.otherPURE: 133377183
dc.identifier.otherPURE UUID: a8087e0a-2b52-49c2-93e4-19421c091543
dc.identifier.otherScopus: 105019395137
dc.identifier.otherWOS: 001569243200001
dc.identifier.urihttp://hdl.handle.net/10362/191005
dc.identifier.urlhttps://www.scopus.com/pages/publications/105019395137
dc.language.isoeng
dc.peerreviewedyes
dc.subjectfracture
dc.subjecthealing
dc.subjectregeneration delay
dc.subjectvascular disruption
dc.subjectVertebral body
dc.subjectSurgery
dc.subjectOrthopedics and Sports Medicine
dc.titleThe healing process of vertebral body fracture in Wistar ratsen
dc.title.subtitlecreation of an animal model and demonstration of the impact of anterolateral vascularization disruption on bone healingen
dc.typejournal article
degois.publication.firstPage526
degois.publication.issue3
degois.publication.lastPage553
degois.publication.titleJournal of Spine Surgery
degois.publication.volume11
dspace.entity.typePublication
person.familyNameCasal
person.familyNameAlves
person.familyNamePAIS
person.givenNameDiogo
person.givenNameSara
person.givenNameDIOGO
person.identifier.ciencia-id301F-64FA-1E13
person.identifier.ciencia-id4613-18B1-4A9C
person.identifier.ciencia-id751C-6525-DD2E
person.identifier.orcid0000-0002-5537-9340
person.identifier.orcid0000-0002-2913-5975
person.identifier.orcid0000-0001-9837-0197
person.identifier.scopus-author-id35434625000
person.identifier.scopus-author-id6603453637
rcaap.rightsopenAccess
relation.isAuthorOfPublication851d9536-1288-403b-b6ad-7282fd830917
relation.isAuthorOfPublication6c9f72f2-6914-4cc4-a08f-270f3c0e78c6
relation.isAuthorOfPublication1701cdec-826d-43c2-a7da-8d6ea20e6638
relation.isAuthorOfPublication.latestForDiscovery6c9f72f2-6914-4cc4-a08f-270f3c0e78c6

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