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Curcumin generates oxidative stress and induces apoptosis in adult schistosoma mansoni worms

2016-11-01Artigo científico Acesso aberto
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dc.contributor.authorDe Aguiar, Daniela Paula
dc.contributor.authorMoscardini, Mayara Brunetto Moreira
dc.contributor.authorMorais, Enyara Rezende
dc.contributor.authorDe Paula, Renato Graciano
dc.contributor.authorFerreira, Pedro Manuel
dc.contributor.authorAfonso, Ana
dc.contributor.authorBelo, Silvana
dc.contributor.authorOuchida, Amanda Tomie
dc.contributor.authorCurti, Carlos
dc.contributor.authorCunha, Wilson Roberto
dc.contributor.authorRodrigues, Vanderlei
dc.contributor.authorMagalhães, Lizandra Guidi
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.pblPLOS - Public Library of Science
dc.date.accessioned2018-05-11T22:05:38Z
dc.date.available2018-05-11T22:05:38Z
dc.date.issued2016-11-01
dc.description.abstractInducing apoptosis is an interesting therapeutic approach to develop drugs that act against helminthic parasites. Researchers have investigated how curcumin (CUR), a biologically active compound extracted from rhizomes of Curcuma longa, affects Schistosoma mansoni and several cancer cell lines. This study evaluates how CUR influences the induction of apoptosis and oxidative stress in couples of adult S. mansoni worms. CUR decreased the viability of adult worms and killed them. The tegument of the parasite suffered morphological changes, the mitochondria underwent alterations, and chromatin condensed. Different apoptotic parameters were determined in an attempt to understand how CUR affected adult S. mansoni worms. CUR induced DNA damage and fragmentation and increased the expression of SmCASP3/7 transcripts and the activity of Caspase 3 in female and male worms. However, CUR did not intensify the activity of Caspase 8 in female or male worms. Evaluation of the superoxide anion and different antioxidant enzymes helped to explore the mechanism of parasite death further. The level of superoxide anion and the activity of Superoxide Dismutase (SOD) increased, whereas the activity of Glutathione-S-Transferase (GST), Glutathione reductase (GR), and Glutathione peroxidase (GPX) decreased, which culminated in the oxidation of proteins in adult female and male worms incubated with CUR. In conclusion, CUR generated oxidative stress followed by apoptotic-like-events in both adult female and male S. mansoni worms, ultimately killing them.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2420236
dc.identifier.doi10.1371/journal.pone.0167135
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 2447741
dc.identifier.otherPURE UUID: 0057031e-c20c-41dd-9ce6-858f8e72923a
dc.identifier.otherScopus: 84996488611
dc.identifier.otherPubMed: 27875592
dc.identifier.otherORCID: /0000-0003-3150-1791/work/40217063
dc.identifier.otherORCID: /0000-0001-8701-8657/work/177738056
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=84996488611&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/84996488611
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGeneral Medicine
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectGeneral Agricultural and Biological Sciences
dc.subjectSDG 3 - Good Health and Well-being
dc.titleCurcumin generates oxidative stress and induces apoptosis in adult schistosoma mansoni wormsen
dc.typejournal article
degois.publication.issue11
degois.publication.titlePLoS ONE
degois.publication.volume11
dspace.entity.typePublication
rcaap.rightsopenAccess

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