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Polyhydroxyalkanoates production in purple phototrophic bacteria ponds

2024-02-20Artigo científico Acesso aberto
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dc.contributor.authorAlmeida, J. R.
dc.contributor.authorLeón, E. Serrano
dc.contributor.authorRogalla, F.
dc.contributor.authorFradinho, J. C.
dc.contributor.authorOehmen, A.
dc.contributor.authorReis, M. A. M.
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.pblElsevier
dc.date.accessioned2024-02-02T23:41:18Z
dc.date.available2024-02-02T23:41:18Z
dc.date.issued2024-02-20
dc.descriptionThis work was supported by national funds from FCT - Fundação para a Ciência e a Tecnologia , I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB . Likewise, the INCOVER project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement n° 689242 ). J.R.A. also acknowledges the financial support of FCT - Fundação para a Ciência e a Tecnologia through the Ph.D. grant DFA/BD/8201/2020. Publisher Copyright: © 2023 The Author(s)
dc.description.abstractThe versatile capacity of purple phototrophic bacteria (PPB) for producing valuable bioproducts has gathered renewed interest in the field of resource recovery and waste valorisation. However, greater knowledge regarding the viability of applying PPB technologies in outdoor, large-scale systems is required. This study assessed, for the first time, the upscaling of the phototrophic polyhydroxyalkanoate (PHA) production technology in a pilot-scale system operated in outdoor conditions. An integrated system composed of two up-flow anaerobic sludge blanket (UASB) reactors (for fermentation of wastewater with molasses), and two high-rate algal ponds retrofitted into PPB ponds, was operated in a wastewater treatment plant under outdoor conditions. UASB's adaptation to the outdoor temperatures involved testing different operational settings, namely hydraulic retention times (HRT) of 48 and 72 h, and molasses fermentation in one or two UASBs. Results have shown that the fermentation of molasses in both UASBs with an increased HRT of 72 h was able to ensure a suitable operation during colder conditions, achieving 3.83 ± 0.63 g CODFermentative Products/L, compared to the 3.73 ± 0.85 g CODFermentative Products/L achieved during warmer conditions (molasses fermentation in one UASB; HRT 48 h). Furthermore, the PPB ponds were operated under a light-feast/dark-aerated-famine strategy and fed with the fermented wastewater and molasses from the two UASBs. The best PHA production was obtained during the summer of 2018 and spring of 2019, attaining 34.7 % gPHA/gVSS with a productivity of 0.11 gPHA L−1 day−1 and 36 % gPHA/gVSS with a productivity of 0.14 gPHA L−1 day−1, respectively. Overall, this study showcases the first translation of phototrophic PHA production technology from an artificially illuminated laboratory scale system into a naturally illuminated, outdoor, pilot-scale system. It also addresses relevant process integration aspects with UASBs for pre-fermenting wastewater with molasses, providing a novel operational strategy to achieve photosynthetic PHA production in outdoor full-scale systems.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent12
dc.format.extent4389384
dc.identifier.doi10.1016/j.scitotenv.2023.168899
dc.identifier.issn0048-9697
dc.identifier.otherPURE: 82176756
dc.identifier.otherPURE UUID: cfe754fd-434f-4d23-b7b1-c189f2c32136
dc.identifier.otherScopus: 85178663796
dc.identifier.otherWOS: 001133555400001
dc.identifier.otherPubMed: 38029992
dc.identifier.urihttp://hdl.handle.net/10362/163062
dc.identifier.urlhttps://www.scopus.com/pages/publications/85178663796
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationInnovative Eco-Technologies for Resource Recovery from Wastewater
dc.subjectOutdoor operation
dc.subjectPilot-scale
dc.subjectPolyhydroxyalkanoate (PHA)
dc.subjectPurple phototrophic bacteria ponds (PPB ponds)
dc.subjectResource recovery
dc.subjectUp-flow anaerobic sludge blanket reactor (UASB)
dc.subjectEnvironmental Engineering
dc.subjectEnvironmental Chemistry
dc.subjectWaste Management and Disposal
dc.subjectPollution
dc.subjectSDG 6 - Clean Water and Sanitation
dc.subjectSDG 9 - Industry, Innovation, and Infrastructure
dc.titlePolyhydroxyalkanoates production in purple phototrophic bacteria pondsen
dc.title.subtitleA breakthrough in outdoor pilot-scale operationen
dc.typejournal article
degois.publication.titleScience of the Total Environment
degois.publication.volume912
dspace.entity.typePublication
oaire.awardNumberUIDB/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardNumber689242
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleInnovative Eco-Technologies for Resource Recovery from Wastewater
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/689242/EU
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccess
relation.isProjectOfPublicatione07cf232-4705-4b5b-b2c4-af8f25311076
relation.isProjectOfPublication834c369e-6900-4105-aa78-6ba0e52f96ca
relation.isProjectOfPublication98e79d76-158b-4b53-8d18-e0bf7282a150
relation.isProjectOfPublication.latestForDiscovery98e79d76-158b-4b53-8d18-e0bf7282a150

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