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DTT protein equalization and Tryptophan protein quantification as a powerful tool in analytical proteomics

2024-04-15Artigo científico Acesso aberto
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dc.contributor.authorDomingos, Inês F.
dc.contributor.authorCarvalho, Luís B.
dc.contributor.authorCapelo, José L.
dc.contributor.authorLodeiro, Carlos
dc.contributor.authorSantos, Hugo M.
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.pblScientific Society Proteomass
dc.date.accessioned2024-09-18T22:22:52Z
dc.date.available2024-09-18T22:22:52Z
dc.date.issued2024-04-15
dc.descriptionPM003/ 2016). This work received support from Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia and Minist\u00E9rio da Ci\u00EAncia, Tecnologia e Ensino Superior (FCT/MCTES) through the projects LA/P/0008/2020 DOI 10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI 10.54499/UIDP/50006/2020 and UIDB/50006/2020 DOI 10.54499/UIDB/50006/2020. H.M.S. acknowledges the Associate Laboratory for Green Chemistry-LAQV (LA/P/0008/2020) DOI 10.54499/LA/P/0008/2020 funded by FCT/ MCTES for his research contract. L.B.C. is supported by the FCT/ MCTES PhD grant 2019 (SFRH/BD/144222/2019). Publisher Copyright: © 2024, Proteomass Scientific Society. All rights reserved.
dc.description.abstractAssessing total protein levels in biological samples is a common procedure in biochemistry and molecular biology. In this study, we compare tryptophan fluorescence (WF) with Bradford and BCA assays to determine total protein in serum samples. Our results indicate that tryptophan fluorescence spectrometry is an efficient, sensitive, and straightforward technique for quantifying proteins in serum. We observed minimal variation between the three methods: BCA de one with the lowers LOD and LOQ. The tryptophan method offers the possibility of reusing the intact sample that does not need colourimetric reagents for quantification. Consequently, free tryptophan serves as a reliable universal standard. This assay can be performed using a conventional fluorescence spectrometer with cuvettes or in a 96-well plate format with a plate reader. The method was successfully used as proof of concept, using serum from patients diagnosed with myeloma and serum from healthy donors.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent5
dc.format.extent1202277
dc.identifier.doi10.5584/jiomics.v14i1.229
dc.identifier.issn2182-0287
dc.identifier.otherPURE: 98898713
dc.identifier.otherPURE UUID: 0eaedd03-ab2d-4321-9175-71a4a7cd9988
dc.identifier.otherScopus: 85192048101
dc.identifier.otherORCID: /0000-0002-6032-8679/work/170346748
dc.identifier.otherORCID: /0000-0001-5582-5446/work/201987765
dc.identifier.urihttp://hdl.handle.net/10362/172032
dc.identifier.urlhttps://www.scopus.com/pages/publications/85192048101
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: PROTEOMASS Scientific Society is acknowledged by the funding provided to the Laboratory for Biological Mass Spectrometry Isabel Moura (
dc.relationPM001/2019 and
dc.relationPM003/ 2016). This work received support from Fundação para a Ciência e a Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES) through the projects LA/P/0008/2020 DOI 10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI 10.54499/UIDP/50006/2020 and UIDB/50006/2020 DOI 10.54499/UIDB/50006/2020. H.M.S. acknowledges the Associate Laboratory for Green Chemistry-LAQV (LA/P/0008/2020) DOI 10.54499/LA/P/0008/2020 funded by FCT/MCTES for his research contract. L.B.C. is supported by the FCT/MCTES PhD grant 2019 (SFRH/BD/144222/2019). Funding Information: PROTEOMASS Scientific Society is acknowledged by the funding provided to the Laboratory for Biological Mass Spectrometry Isabel Moura (
dc.relationPM001/2019 and
dc.subjectDTT equalization
dc.subjectmultiple myeloma
dc.subjectproteomics
dc.subjectserum
dc.subjecttryptophan
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectGenetics
dc.titleDTT protein equalization and Tryptophan protein quantification as a powerful tool in analytical proteomicsen
dc.typejournal article
degois.publication.firstPage7
degois.publication.issue1
degois.publication.lastPage11
degois.publication.titleJournal of Integrated OMICS
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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