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Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes

2008-08-22Artigo científico Acesso aberto
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dc.contributor.authorFigueiredo, Ana C.
dc.contributor.authorWasmeier, Christina
dc.contributor.authorTarafder, Abul K.
dc.contributor.authorRamalho, José S.
dc.contributor.authorBaron, Rudi A.
dc.contributor.authorSeabra, Miguel C.
dc.contributor.authorC Seabra, Miguel
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblASBMB - American Society for Biochemistry and Molecular Biology
dc.date.accessioned2022-07-19T22:20:38Z
dc.date.available2022-07-21T00:31:29Z
dc.date.embargoedUntil2022-07-20
dc.date.issued2008-08-22
dc.description.abstractRab GTPases regulate discrete steps in vesicular transport pathways. Rabs require activation by specific guanine nucleotide exchange factors (GEFs) that stimulate the exchange of GDP for GTP. Rab27a controls motility and regulated exocytosis of secretory granules and related organelles. In melanocytes, Rab27a regulates peripheral transport of mature melanosomes by recruiting melanophilin and myosin Va. Here, we studied the activation of Rab27a in melanocytes. We identify Rab3GEP, previously isolated as a GEF for Rab3a, as the non-redundant Rab27a GEF. Similar to Rab27a-deficient ashen melanocytes, Rab3GEP-depleted cells show both clustering of melanosomes in the perinuclear area and loss of the Rab27a effector Mlph. Consistent with a role as an activator, levels of Rab27a-GTP are decreased in cells lacking Rab3GEP. Recombinant Rab3GEP exhibits guanine nucleotide exchange activity against Rab27a and Rab27b in vitro, in addition to its previously documented activity against Rab3. Our results indicate promiscuity in Rab GEF action and suggest that members of related but functionally distinct Rab subfamilies can be controlled by common activators.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent8
dc.format.extent868253
dc.identifier.doi10.1074/jbc.M804134200
dc.identifier.issn0021-9258
dc.identifier.otherPURE: 3202996
dc.identifier.otherPURE UUID: 2eb9927d-1e30-4e1d-a557-5b6977845042
dc.identifier.otherScopus: 53149101728
dc.identifier.otherPubMed: 18559336
dc.identifier.otherWOS: 000258455400034
dc.identifier.urihttp://hdl.handle.net/10362/142153
dc.identifier.urlhttps://www.scopus.com/pages/publications/53149101728
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectCell Biology
dc.titleRab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytesen
dc.typejournal article
degois.publication.firstPage23209
degois.publication.issue34
degois.publication.lastPage23216
degois.publication.titleJournal of Biological Chemistry
degois.publication.volume283
dspace.entity.typePublication
person.familyNameSeabra
person.givenNameMiguel
person.identifier.ciencia-id2512-FAEF-E26F
person.identifier.orcid0000-0002-6404-4892
person.identifier.ridM-3280-2013
person.identifier.scopus-author-id7004369652
rcaap.rightsopenAccess
relation.isAuthorOfPublicationd6eeb095-df2d-4fd0-8241-ab826e5ecb1d
relation.isAuthorOfPublication.latestForDiscoveryd6eeb095-df2d-4fd0-8241-ab826e5ecb1d

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