Publicação
Impact of chromossomal structure on the evolution of Schizosaccharomyces pombe undergoing Mutation Accumulation
| datacite.subject.fos | Engenharia e Tecnologia | pt_PT |
| dc.contributor.advisor | Perfeito, Lília | |
| dc.contributor.author | Eduardo, Gustavo Nuno Martins | |
| dc.date.accessioned | 2016-02-12T10:39:39Z | |
| dc.date.available | 2016-02-12T10:39:39Z | |
| dc.date.issued | 2015-10 | |
| dc.date.submitted | 2016-02 | |
| dc.description.abstract | Large chromosomal rearrangements are common in natural populations and thought to be involved in speciation events. In this project, we used experimental evolution to determine how the speed of evolution and the type of accumulated mutations depend on the ancestral chromosomal structure and genotype. We utilized two Wild Type strains and a set of genetically engineered Schizosaccharomyces pombe strains, different solely in the presence of a certain type of chromosomal variant (inversions or translocations), along with respective controls. Previous research has shown that these chromosomal variants have different fitness levels in several environments, probably due to changes in the gene expression along the genome. These strains were propagated in the laboratory at very low population sizes, in which we expect natural selection to be less efficient at purging deleterious mutations. We then measured these strains’ changes in fitness throughout this accumulation of deleterious mutations, comparing the evolutionary trajectories in the different rearrangements to understand if the chromosomal structure affected the speed of evolution. We also tested these mutations for possible epistatic effects and estimated their parameters: the number of arising deleterious mutations per generation (Ud) and each one’s mean effect (sd). | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10362/16444 | |
| dc.language.iso | eng | pt_PT |
| dc.subject | Schizosaccharomyces pombe | pt_PT |
| dc.subject | Chromosomal rearrangements | pt_PT |
| dc.subject | Mutation accumulation | pt_PT |
| dc.subject | Recombination | pt_PT |
| dc.subject | Evolution | pt_PT |
| dc.title | Impact of chromossomal structure on the evolution of Schizosaccharomyces pombe undergoing Mutation Accumulation | pt_PT |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | masterThesis | pt_PT |
| thesis.degree.name | Mestrado em Genética Molecular e Biomedicina | pt_PT |