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An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care

2022-12Artigo científico Acesso aberto
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dc.contributor.authorCardoso, Ana R.
dc.contributor.authorAlves, João Frederico
dc.contributor.authorFrasco, Manuela F.
dc.contributor.authorPiloto, Ana Margarida
dc.contributor.authorSerrano, Verónica
dc.contributor.authorMateus, Daniela
dc.contributor.authorSebastião, Ana Isabel
dc.contributor.authorMatos, Ana Miguel
dc.contributor.authorCarmo, Anália
dc.contributor.authorCruz, Teresa
dc.contributor.authorFortunato, Elvira
dc.contributor.authorSales, M. Goreti F.
dc.contributor.institutionCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
dc.contributor.institutionDCM - Departamento de Ciência dos Materiais
dc.contributor.institutionUNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
dc.contributor.pblElsevier BV
dc.date.accessioned2023-03-10T22:54:24Z
dc.date.available2023-03-10T22:54:24Z
dc.date.issued2022-12
dc.descriptionFunding Information: The authors acknowledge funding through project TecniCov ( POCI-01-02B7-FEDER-069745 ), co-funded by FEDER through COMPETE2020 and Lisboa2020 and CY-Sensors ( POCI-01-0145-FEDER-032359 ) through Fundação para a Ciência e a Tecnologia (FCT) , Portugal. Publisher Copyright: © 2022 The Authors
dc.description.abstractThis work presents an innovative ultra-sensitive biosensor having the Spike protein on carbon-based screen-printed electrodes (SPEs), for monitoring in point-of-care antibodies against SARS-CoV-2, a very important tool for epidemiological monitoring of COVID-19 infection and establishing vaccination schemes. In an innovative and simple approach, a highly conductive support is combined with the direct adsorption of Spike protein to enable an extensive antibody capture. The high conductivity was ensured by using carboxylated carbon nanotubes on the carbon electrode, by means of a simple and quick approach, which also increased the surface area. These were then modified with EDC/NHS chemistry to produce an amine layer and undergo Spike protein adsorption, to generate a stable layer capable of capturing the antibodies against SARS-CoV-2 in serum with great sensitivity. Electrochemical impedance spectroscopy was used to evaluate the analytical performance of this biosensor in serum. It displayed a linear response between 1.0 ​pg/mL and 10 ​ng/mL, with a detection limit of ∼0.7 ​pg/mL. The analysis of human positive sera containing antibody in a wide range of concentrations yielded accurate data, correlating well with the reference method. It also offered the unique ability of discriminating antibody concentrations in sera below 2.3 ​μg/mL, the lowest value detected by the commercial method. In addition, a proof-of-concept study was performed by labelling anti-IgG antibodies with quantum dots to explore a new electrochemical readout based on the signal generated upon binding to the anti-S protein antibodies recognised on the surface of the biosensor. Overall, the alternative serologic assay presented is a promising tool for assessing protective immunity to SARS-CoV-2 and a potential guide for revaccination.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent10
dc.format.extent1492101
dc.identifier.doi10.1016/j.mtbio.2022.100354
dc.identifier.issn2590-0064
dc.identifier.otherPURE: 55265736
dc.identifier.otherPURE UUID: 1b1a930d-8be0-40da-b7c6-e61709fc07ec
dc.identifier.otherScopus: 85134611987
dc.identifier.otherWOS: 000843481000009
dc.identifier.otherPubMed: 35847374
dc.identifier.otherPubMedCentral: PMC9270181
dc.identifier.otherORCID: /0000-0002-4202-7047/work/130607700
dc.identifier.urihttp://hdl.handle.net/10362/150359
dc.identifier.urlhttps://www.scopus.com/pages/publications/85134611987
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F130107%2F2017/PT
dc.relationalterado para: “NANOZYME+ / Driving nanozymes towards stereochemical recognition: application to biomolecules of interest in health”; NANOZYME+ / Driving nanozymes towards stereochemical recognition: application to Glucose sensing
dc.subjectAntibodies for SARS-CoV-2
dc.subjectElectrochemical biosensor
dc.subjectPoint-of-care
dc.subjectProtective immunity
dc.subjectSARS-CoV-2
dc.subjectSpike protein
dc.subjectBiotechnology
dc.subjectBioengineering
dc.subjectBiomaterials
dc.subjectBiomedical Engineering
dc.subjectMolecular Biology
dc.subjectCell Biology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleAn ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-careen
dc.typejournal article
degois.publication.titleMaterials Today Bio
degois.publication.volume16
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/130107/2017
oaire.awardTitlealterado para: “NANOZYME+ / Driving nanozymes towards stereochemical recognition: application to biomolecules of interest in health”; NANOZYME+ / Driving nanozymes towards stereochemical recognition: application to Glucose sensing
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F130107%2F2017/PT
oaire.fundingStreamPOR_NORTE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublicationfe7031a1-39b5-43fd-864b-534a60bfe8d1
relation.isProjectOfPublication.latestForDiscoveryfe7031a1-39b5-43fd-864b-534a60bfe8d1

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