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Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition

2019-10-01Artigo científico Acesso aberto
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dc.contributor.authorDelgado, Veronica Palma
dc.contributor.authorPaquete, Catarina M.
dc.contributor.authorSturm, Gunnar
dc.contributor.authorGescher, Johannes
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblElsevier Science B.V., Amsterdam.
dc.date.accessioned2020-01-17T23:34:18Z
dc.date.available2024-01-01T01:31:47Z
dc.date.embargoedUntil2023-12-31
dc.date.issued2019-10-01
dc.description.abstractPeriplasmic c-type cytochromes are essential for the electron transport between the cytoplasmic membrane bound menquinol oxidase CymA and the terminal ferric iron reductase MtrABC in the outer membrane of Shewanella oneidensis cells. Either STC or FccA are necessary for periplasmic electron transfer. We followed the hypothesis that the elimination of potential competing reactions in the periplasm and the simultaneous overexpression of STC (cctA)could lead to an accelerated electron transfer to the cell surface. The genes nrfA, ccpA, napB and napA were replaced by cctA. This led to a 1.7-fold increased ferric iron reduction rate and a 23% higher current generation in a bioelectrochemical system. Moreover, the quadruple mutant had a higher periplasmic flavin content. Further deletion of fccA and its replacement by cctA resulted in a strain with ferric iron reduction rates similar to the wild type and a lower concentration of periplasmic flavin compared to the quadruple mutant. A transcriptomic analysis revealed that the quadruple mutant had a 3.7-fold higher cctA expression which could not be further increased by the replacement of fccA. This work indicates that a synthetic adaptation of Shewanella towards extracellular respiration holds potential for increased respiratory rates and consequently higher current densities.en
dc.description.versionauthorsversion
dc.description.versionpublished
dc.format.extent8
dc.format.extent2773461
dc.identifier.doi10.1016/j.bioelechem.2019.04.022
dc.identifier.issn1567-5394
dc.identifier.otherPURE: 13702085
dc.identifier.otherPURE UUID: ef22867f-4d7e-4adb-9de5-d8e900315ee0
dc.identifier.otherScopus: 85065206585
dc.identifier.otherPubMed: 31075535
dc.identifier.otherORCID: /0000-0002-1511-3732/work/68290018
dc.identifier.urihttp://hdl.handle.net/10362/91391
dc.identifier.urlhttps://www.scopus.com/pages/publications/85065206585
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBioelectrochemical system
dc.subjectCurrent density
dc.subjectElectron transfer
dc.subjectGenetic engineering
dc.subjectPeriplasmic cytochrome
dc.subjectShewanella oneidensis
dc.subjectBiophysics
dc.subjectPhysical and Theoretical Chemistry
dc.subjectElectrochemistry
dc.titleImprovement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein compositionen
dc.typejournal article
degois.publication.firstPage18
degois.publication.lastPage25
degois.publication.titleBioelectrochemistry
degois.publication.volume129
dspace.entity.typePublication
rcaap.rightsopenAccess

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