Logo do repositório
 
Publicação

Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children

2022-06Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorAlenquer, Marta
dc.contributor.authorSilva, Tiago Milheiro
dc.contributor.authorAkpogheneta, Onome
dc.contributor.authorFerreira, Filipe
dc.contributor.authorVale-Costa, Sílvia
dc.contributor.authorMedina-Lopes, Mónica
dc.contributor.authorBatista, Frederico
dc.contributor.authorGarcia, Ana Margarida
dc.contributor.authorBarreto, Vasco M.
dc.contributor.authorPaulino, Cathy
dc.contributor.authorCosta, João
dc.contributor.authorSobral, João
dc.contributor.authorDiniz-Da-Costa, Maria
dc.contributor.authorLadeiro, Susana
dc.contributor.authorCorte-Real, Rita
dc.contributor.authorAlves, José Delgado
dc.contributor.authorLeite, Ricardo B.
dc.contributor.authorDemengeot, Jocelyne
dc.contributor.authorBrito, Maria João Rocha
dc.contributor.authorAmorim, Maria João
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.pblPLOS - Public Library of Science
dc.date.accessioned2022-07-18T22:27:08Z
dc.date.available2022-07-18T22:27:08Z
dc.date.issued2022-06
dc.descriptionFunding Information: This work was funded by the Fundação para a Ciência and Tecnologia (FCT, Portugal) under RESEARCH4COVID 19 call with reference 283_596885654 and co-funded by ANI under INOV4COVID (Funding to V.M.B.). M.J.A. is funded by the FCT (CEECIND/02373/2020). M.A. is funded by a Junior Researcher working contract from FCT and Instituto Gulbenkian de Ciência (IGC, Portugal). This work benefited from COVID19 emergency funds 2020 from Calouste Gulbenkian Foundation and from Oeiras city council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We would like to thank the technical support of IGC’s Advanced Imaging Facility (AIF-UIC).
dc.description.abstractBackground Adults are being vaccinated against SARS-CoV-2 worldwide, but the longitudinal protection of these vaccines is uncertain, given the ongoing appearance of SARS-CoV-2 variants. Children remain largely unvaccinated and are susceptible to infection, with studies reporting that they actively transmit the virus even when asymptomatic, thus affecting the community. Methods We investigated if saliva is an effective sample for detecting SARS-CoV-2 RNA and antibodies in children, and associated viral RNA levels to infectivity. For that, we used a saliva-based SARS-CoV-2 RT-qPCR test, preceded or not by RNA extraction, in 85 children aged 10 years and under, admitted to the hospital regardless of COVID-19 symptomatology. Amongst these, 29 (63.0%) presented at least one COVID-19 symptom, 46 (54.1%) were positive for SARS-CoV-2 infection, 28 (32.9%) were under the age of 1, and the mean (SD) age was 3.8 (3.4) years. Saliva samples were collected up to 48 h after a nasopharyngeal swab-RT-qPCR test. Results In children aged 10 years and under, the sensitivity, specificity, and accuracy of saliva-RT-qPCR tests compared to NP swab-RT-qPCR were, respectively, 84.8% (71.8%–92.4%), 100% (91.0%–100%), and 91.8% (84.0%–96.6%) with RNA extraction, and 81.8% (68.0%–90.5%), 100% (91.0%–100%), and 90.4% (82.1%–95.0%) without RNA extraction. Rescue of infectious particles from saliva was limited to CT values below 26. In addition, we found significant IgM positive responses to SARS-CoV-2 in children positive for SARS-CoV-2 by NP swab and negative by saliva compared to other groups, indicating late infection onset (>7–10 days). Conclusions Saliva is a suitable sample type for diagnosing children aged 10 years and under, including infants aged <1 year, even bypassing RNA extraction methods. Importantly, the detected viral RNA levels were significantly above the infectivity threshold in several samples. Further investigation is required to correlate SARS-CoV-2 RNA levels to viral transmission.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1169425
dc.identifier.doi10.1371/journal.pone.0268388
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 45372087
dc.identifier.otherPURE UUID: f7ea860f-f64c-421b-8ccc-bbd8ef8cb391
dc.identifier.otherScopus: 85132082722
dc.identifier.otherPubMed: 35704567
dc.identifier.otherWOS: 000843619700132
dc.identifier.urihttp://hdl.handle.net/10362/142070
dc.identifier.urlhttps://www.scopus.com/pages/publications/85132082722
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGeneral
dc.subjectSDG 3 - Good Health and Well-being
dc.titleSaliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in childrenen
dc.typejournal article
degois.publication.issue6 June
degois.publication.titlePLoS ONE
degois.publication.volume17
dspace.entity.typePublication
rcaap.rightsopenAccess

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
journal.pone.0268388.pdf
Tamanho:
1.12 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

NMS: CEDOC - Artigos em revista internacional com arbitragem científica