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Genetic diversity and drug resistance of HIV-1 among infected pregnant women newly diagnosed in Luanda, Angola

2019-11-26Artigo científico Acesso aberto
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dc.contributor.authorSebastião, Cruz S
dc.contributor.authorNeto, Zoraima
dc.contributor.authorde Jesus, Carlos S
dc.contributor.authorMirandela, Marinela
dc.contributor.authorJandondo, Domingos
dc.contributor.authorCouto-Fernandez, José C
dc.contributor.authorTanuri, Amilcar
dc.contributor.authorMorais, Joana
dc.contributor.authorBrito, Miguel
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblPLOS - Public Library of Science
dc.date.accessioned2020-04-14T22:34:58Z
dc.date.available2020-04-14T22:34:58Z
dc.date.issued2019-11-26
dc.description.abstractMonitoring genetic diversity and drug resistance mutations (DRMs) is critical for understanding HIV epidemiology. Here, we report HIV-1 genetic diversity and DRMs in blood samples from 42 HIV-positive pregnant women naive to antiretroviral therapy (ART), in Luanda. The samples were subjected to nested-PCR, followed by sequencing of HIV-1 pol gene, targeting the protease and reverse transcriptase fragments. HIV-1 diversity was analyzed using the REGA HIV-1 subtyping tool and DRMs were identified using the Calibrated Population Resistance tool. A total of 34 sequences were obtained. The data revealed wide HIV-1 subtypes heterogeneity, with subtype C (38%, 13/34) the most frequent, followed by the subtypes F1 (18%, 6/34), A1 (9%, 3/34), G (9%, 3/34), D (6%, 2/34) and H (3%, 1/34). In addition, recombinants strains were detected, with CRF02_AG (6%, 2/34) the most frequent, followed by CRF37_cpx, F1/C, A1/G and H/G, all with 3% (1/34). A total of 6/34 (18%) of the sequences presented DRMs. The non-nucleoside reverse transcriptase inhibitors presented 15% (5/34) of resistance. Moreover, 1/34 (3%) sequence presented resistance against both non-nucleoside reverse transcriptase inhibitors and nucleoside reverse transcriptase inhibitors, simultaneously. Despite the small sample size, our results suggest the need to update currently used ART regimens. Surveillance of HIV-1 subtypes and DRMs are necessary to understand HIV epidemiology and to guide modification of ART guidelines in Angola.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent916168
dc.identifier.doi10.1371/journal.pone.0225251
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 17643399
dc.identifier.otherPURE UUID: b6b83915-b024-4d19-ab1a-b2a30f180fce
dc.identifier.otherPubMed: 31770425
dc.identifier.otherPubMedCentral: PMC6879122
dc.identifier.otherScopus: 85075618640
dc.identifier.otherWOS: 000533892300012
dc.identifier.urihttp://hdl.handle.net/10362/96168
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAngola
dc.subjectAnti-HIV Agents/pharmacology
dc.subjectCross-Sectional Studies
dc.subjectDrug Resistance, Viral
dc.subjectFemale
dc.subjectGenetic Variation
dc.subjectGenotyping Techniques
dc.subjectHIV Infections/diagnosis
dc.subjectHIV-1/classification
dc.subjectHumans
dc.subjectPhylogeny
dc.subjectPopulation Surveillance
dc.subjectPregnancy
dc.subjectPregnancy Complications, Infectious/virology
dc.subjectSample Size
dc.subjectSequence Analysis, RNA/methods
dc.subjectYoung Adult
dc.subjectpol Gene Products, Human Immunodeficiency Virus/genetics
dc.subjectSDG 3 - Good Health and Well-being
dc.titleGenetic diversity and drug resistance of HIV-1 among infected pregnant women newly diagnosed in Luanda, Angolaen
dc.typejournal article
degois.publication.issue11
degois.publication.titlePLoS ONE
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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