A carregar...
Projeto de investigação
Sem título
Financiador
Autores
Publicações
Optimizing magnetic hyperthermia for melanoma
Publication . Simões, Beatriz T.; Chaparro, Catarina; Vieira, Tânia; Valente, Manuel A.; Cavaco, Marco C. V.; Neves, Vera; Silva, Jorge C.; Borges, João Paulo; Almeida, Filipe V.; Soares, Paula; DCM - Departamento de Ciência dos Materiais; CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N); DF – Departamento de Física; IOP Publishing
Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used in magnetic hyperthermia, where their therapeutic efficacy depends on efficient heat generation. However, intracellular uptake of SPIONs has been shown to reduce their heat dissipation capacity, limiting hyperthermia performance. To address this challenge, we explored the use of small-molecule endocytosis inhibitors to block SPIONs’ uptake in vitro. SPIONs stabilized with 3-aminopropyl triethoxysilane (APTES) were evaluated in an advanced cutaneous melanoma cell line treated with a small library of endocytosis inhibitors. Among these, methyl-β-cyclodextrin significantly reduced SPIONs’ uptake compared to untreated cells. Importantly, uptake inhibition restored SPIONs’ heat dissipation capacity from specific absorption rates of 63 to 91 W g−1 and improved the temperature increase by 2.6 °C, under magnetic hyperthermia conditions. These findings demonstrate that targeting nanoparticle internalization with small-molecule inhibitors, particularly methyl-β-cyclodextrin, enhances the efficiency of magnetic hyperthermia in melanoma cells. This strategy offers a promising approach to optimize magnetic hyperthermia for melanoma treatment.
Unidades organizacionais
Descrição
Palavras-chave
Contribuidores
Financiadores
Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
OE
Número da atribuição
SFRH/BD/148588/2019