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Projeto de investigação
Development of adjustable Silver@Silica nanocarriers and their stabilization in solid materials against drug resistant and non-resistant pathogenic bacteria. Towards advanced nano-antibiotics, wound dressing and anti-biofilm surfaces
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Adenosine-Monophosphate-Assisted Homogeneous Silica Coating of Silver Nanoparticles in High Yield
Publication . Fernández-Lodeiro, Carlos; Tambosi, Reem; Fernández-Lodeiro, Javier; Fernández-Lodeiro, Adrián; Nuti, Silvia; Ouchane, Soufian; Kébaïli, Nouari; Pérez-Juste, Jorge; Pastoriza-Santos, Isabel; Lodeiro, Carlos; DQ - Departamento de Química; LAQV@REQUIMTE; MDPI AG
In this study, we propose a novel approach for the silica coating of silver nanoparticles based on surface modification with adenosine monophosphate (AMP). Upon AMP stabilization, the nanoparticles can be transferred into 2-propanol, promoting the growth of silica on the particle surfaces through the standard Stöber process. The obtained silica shells are uniform and homogeneous, and the method allows a high degree of control over shell thickness while minimizing the presence of uncoated NPs or the negligible presence of core-free silica NPs. In addition, AMP-functionalized AgNPs could be also coated with a mesoporous silica shell using cetyltrimethylammonium chloride (CTAC) as a template. Interestingly, the thickness of the mesoporous silica coating could be tightly adjusted by either the silica precursor concentration or by varying the CTAC concentration while keeping the silica precursor concentration constant. Finally, the influence of the silica coating on the antimicrobial effect of AgNPs was studied on Gram-negative bacteria (R. gelatinosus and E. coli) and under different bacterial growth conditions, shedding light on their potential applications in different biological environments.
Synthesis of mesoporous silica coated gold nanorods loaded with methylene blue and its potentials in antibacterial applications
Publication . Fernández-Lodeiro, Adrián; Djafari, Jamila; Fernández-Lodeiro, Javier; Duarte, Maria Paula; Mauricio, Elisabete Muchagato; Capelo-Martínez, José Luis; Lodeiro, Carlos; LAQV@REQUIMTE; DQ - Departamento de Química; MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos; DCTB - Departamento de Ciências e Tecnologia da Biomassa (ex-GDEH); MDPI AG
In this work, the successful preparation and characterization of gold nanorods (AuNRs) coated with a mesoporous silica shell (AuNRs@Simes) was achieved. Conjugation with methylene blue (MB) as a model drug using ultrasound-stimulated loading has been explored for further application in light-mediated antibacterial studies. Lyophilization of this conjugated nanosystem was analyzed using trehalose (TRH) as a cryogenic protector. The obtained stable dry formulation shows potent antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria after a simple post-treatment irradiation method with a red laser during a short time period.
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Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
3599-PPCDT
Número da atribuição
PTDC/QUI-COL/1517/2020