Freire, FilipeRomão, Maria JoãoMacEdo, Anjos L.Aveiro, Susana S.Goodfellow, Brian J.Carvalho, Ana Luísa2019-03-112019-03-112009-01-011744-3091PURE: 12011100PURE UUID: a3aaaaf8-7787-43e1-8cf9-4d87e6199ed6Scopus: 68149089746PubMed: 19574650PubMedCentral: PMC2705645WOS: 000267530000018ORCID: /0000-0002-3004-0543/work/55386355ORCID: /0000-0002-3824-0240/work/55386447http://www.scopus.com/inward/record.url?scp=68149089746&partnerID=8YFLogxKThe authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia, Portugal through grant SFRH/BPD/30239/2006. The authors also acknowledge the ESRF, Grenoble (beamlines ID14- EH2, ID14- EH4, ID23- EH1 and ID-29) for access and technical support during data collection.Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6422, with unit-cell parameters a = b = 145, c = 60 Å and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 Å resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.4242672engHaem-binding proteinsSOULBiophysicsStructural BiologyBiochemistryGeneticsCondensed Matter Physicsjournal article10.1107/S174430910902291Xhttps://www.scopus.com/pages/publications/68149089746