Gallão, Maria I.Cortelazzo, Angelo L.Fevereiro, Manoel P.S.De Brito, Edy S.2025-12-032025-12-0320071677-0420PURE: 33755180PURE UUID: 808a266b-0f5a-446a-9930-d35c132d1a76Scopus: 46349092149http://hdl.handle.net/10362/191453Morphological changes and enzyme activity in suspension-cultured cells of Citrus aurantium were followed after chitin elicitation. Chitin oligomers presented a rapid effect with a maximum activity after 3 h followed by a gradual decrease to the original levels at 8 h of incubation. Cell walls presented a lignification process and the cytoplasmatic protein became less reactive to anionic stains. In the treated material a reduction in starch grain size, an increase in the number of autophagic vacuoles, deposition of secretion in the pericellular space and a defibrillation of the cell-wall polymers were observed. Chitin oligomers increased peroxidase and L-phenylalanine ammonia-lyase activities. Extracellular peroxidase activity increased from 0.20 U mL -1 after 1 h to 0.45 U mL-1 at 3 h followed by a gradual decrease up to 8 h. The peroxidase isoenzyme pattern revealed a predominance of basic isoenzymes in these cells.8314433engChitinCitrus aurantiumElicitationL-phenylalanine ammonia-lyasePeroxidaseAgronomy and Crop SciencePlant Sciencejournal article10.1590/s1677-04202007000100008https://www.scopus.com/pages/publications/46349092149