Silva, Marcelo2021-05-042021-05-042013-01-012314-6133PURE: 169908PURE UUID: 0cb3e4d5-7bf1-4502-af69-43f18c309692researchoutputwizard: 44211PubMed: 23710438Scopus: 84877307381WOS: 000317495700001http://hdl.handle.net/10362/116942Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.1320228undSDG 3 - Good Health and Well-beingMolecular techniques for dicistrovirus detection without RNA extraction or purification.journal article10.1155/2013/218593