Domingos, PedroDias Santos, Cristiana Catarina2025-07-312025-07-072025-03-10http://hdl.handle.net/10362/185811"The accumulation of misfolded/unfolded proteins in the endoplasmic reticulum (ER) leads to a cellular condition known as “ER stress”. To alleviate this stress and restore cellular homeostasis, cells activate a signalling pathway collectively referred to as the Unfolded Protein Response (UPR). As a result, the ER sensor protein IRE1 is activated and mediates the splicing of XBP1 mRNA. Xbp1 spliced (Xbp1s) is an effective transcription factor and promotes the transcription of UPR-target genes, helping to reduce ER stress levels. However, when stress is prolonged or severe, it can lead to cell death by apoptosis.(...)"engFbxo42 geneRbx (SCF complexes)HEK-293 cellAtaxin-2Xbp1 mRNAdoctoral thesis101827024