Dias, Carolina Gomes Ferreira2024-02-062024-10-312023-01-192022-10-01http://hdl.handle.net/10362/163175"Galactose oxidases (GalOxs) are copper radical oxidases (CROs) that catalyze the oxidation of primary alcohols to the corresponding aldehydes, reducing molecular oxygen to hydrogen peroxide. These monomeric metalloenzymes have a copper ion as a cofactor and are secreted by filamentous fungi, with FgrGalOx being the best-studied representative. GalOxs holds potential for various biotechnological applications in small molecule synthesis, oxygen removal, and biosensors. Therefore, a better understanding of GalOx from different organisms is crucial, as it may reveal novel or improved properties to be explored in various fields. This work unravels the properties of a new bacterial galactose oxidase, AsGalOx, from Pseudoarthrobacter siccitolerans while simultaneously tailoring its properties through enzyme engineering approaches.(...)"engradical copper oxidasesgalactose oxidasedirected evolutioncatalytic efficiencymaster thesis203518640