Marques, IsabelRodrigues, Ana P.Gouveia, DuarteLidon, Fernando C.Martins, SóniaSemedo, Magda C.Gaillard, Jean CharlesPais, Isabel P.Semedo, José N.Scotti-Campos, PaulaReboredo, Fernando H.Partelli, Fábio L.DaMatta, Fábio M.Armengaud, JeanRibeiro-Barros, Ana I.Ramalho, José C.2022-11-282022-11-282022-090176-1617PURE: 47635023PURE UUID: 6607cf2c-eae2-42af-8dc6-c898db236535Scopus: 85136633980PubMed: 35944291WOS: 000847851300005ORCID: /0000-0002-3906-4349/work/123728529http://hdl.handle.net/10362/145852This work received funding support from the European Union's Horizon 2020 research and innovation program (grant agreement No 727934 , project BreedCAFS), ( CEF ), - Individual Call (CEEC Individual) - 2021.01107.CEECIND/CP1689/CT0001 (IM) . Fellowships from the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq) , (to F.M. DaMatta and F.L. Partelli), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG, projects CRA-RED-00053-16 and APQ 01512-18 , to F.M. DaMatta) are also greatly acknowledged. Publisher Copyright: © 2022 Elsevier GmbHAs drought threatens crop productivity it is crucial to characterize the defense mechanisms against water deficit and unveil their interaction with the expected rise in the air [CO2]. For that, plants of Coffea canephora cv. Conilon Clone 153 (CL153) and C. arabica cv. Icatu grown under 380 (aCO2) or 700 μL L−1 (eCO2) were exposed to moderate (MWD) and severe (SWD) water deficits. Responses were characterized through the activity and/or abundance of a selected set of proteins associated with antioxidative (e.g., Violaxanthin de-epoxidase, Superoxide dismutase, Ascorbate peroxidases, Monodehydroascorbate reductase), energy/sugar (e.g., Ferredoxin-NADP reductase, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, sucrose synthase, mannose-6-phosphate isomerase, Enolase), and lipid (Lineolate 13S-lipoxygenase) processes, as well as with other antioxidative (ascorbate) and protective (HSP70) molecules. MWD caused small changes in both genotypes regardless of [CO2] level while under the single imposition to SWD, only Icatu showed a global reinforcement of most studied proteins supporting its tolerance to drought. eCO2 alone did not promote remarkable changes but strengthened a robust multi-response under SWD, even supporting the reversion of impacts already observed by CL153 at aCO2. In the context of climate changes where water constraints and [CO2] levels are expected to increase, these results highlight why eCO2 might have an important role in improving drought tolerance in Coffea species.142000733engAcclimationAntioxidative responseClimate changeCoffee treeElevated air [CO2]HSP70Proteomic analysisPhysiologyAgronomy and Crop SciencePlant ScienceSDG 13 - Climate Actionjournal article10.1016/j.jplph.2022.153788https://www.scopus.com/pages/publications/85136633980