Mondal, SuchintanAlke, Bhavnade Castro, Aline MachadoOrtiz-Albo, PalomaSyed, Usman TaquiCrespo, João G.Brazinha, Carla2022-11-292022-11-292022-08-180076-6356PURE: 46771658PURE UUID: 3ccd7a97-0c7a-43b3-a3f3-fea71e14059dScopus: 85137354893WOS: 000845542000001PubMed: 36005712PubMedCentral: PMC9416194ORCID: /0000-0001-8714-3781/work/123810587ORCID: /0000-0002-7784-0265/work/123810722http://hdl.handle.net/10362/145887Publisher Copyright: © 2022 by the authors.Membrane-based gas separation is a promising unit operation in a low-carbon economy due to its simplicity, ease of operation, reduced energy consumption and portability. A methodology is proposed to immobilise enzymes in stable water-in-oil (W/O) emulsions produced by direct membrane emulsification systems and thereafter impregnated them in the pores of a membrane producing emulsion-based supported liquid membranes. The selected case-study was for biogas (CO2 and CH4) purification. Upon initial CO2 sorption studies, corn oil was chosen as a low-cost and non-toxic bulk phase (oil phase). The emulsions were prepared with Nadir® UP150 P flat-sheet polymeric membranes. The optimised emulsions consisted of 2% Tween 80 (w/w) in corn oil as the continuous phase and 0.5 g.L−1 carbonic anhydrase enzyme with 5% PEG 300 (w/w) in aqueous solution as the dispersed phase. These emulsions were impregnated onto a porous hydrophobic PVDF membrane to prepare a supported liquid membrane for gas separation. Lastly, gas permeability studies indicated that the permeability of CO2 increased by ~15% and that of CH4 decreased by ~60% when compared to the membrane without carbonic anhydrase. Thus, a proof-of-concept for enhancement of CO2 capture using emulsion-based supported liquid membrane was established.155386333engcarbonic anhydraseCO recovery from biogasemulsion-based supported liquid membranemembrane emulsificationwater-in-oil emulsionsChemical Engineering (miscellaneous)Process Chemistry and TechnologyFiltration and SeparationSDG 7 - Affordable and Clean EnergySDG 8 - Decent Work and Economic GrowthSDG 12 - Responsible Consumption and ProductionSDG 13 - Climate Actionjournal article10.3390/membranes12080797https://www.scopus.com/pages/publications/85137354893