Weng, VerónicaCardeira, MartimBento-Silva, AndreiaSerra, Ana TeresaBrazinha, CarlaBronze, Maria Rosário2023-11-102023-11-102023-07-251420-3049PURE: 75819421PURE UUID: ccbce23b-18e4-4c40-8f9e-11b3aeb2dd4cScopus: 85167749418WOS: 001045602500001PubMed: 37570593PubMedCentral: PMC10420191ORCID: /0000-0002-7784-0265/work/151431934http://hdl.handle.net/10362/159824Funding Information: The authors acknowledge the financial support from the Fundação para a Ciência e Tecnologia and Portugal 2020 to the Portuguese Mass Spectrometry Network (LISBOA-01-0145-FEDER-402-022125). This research was also funded by the Sociedade Ponto Verde—Sociedade de Resíduos de Embalagens, S.A., through the project entitled “Bioplastics and Edible, Vegan Films”. Publisher Copyright: © 2023 by the authors.Arabinoxylan has prebiotic properties, as it is able to resist digestion in the small intestine and undergoes fermentation in the large intestine. In this work, arabinoxylan was extracted from corn fiber using an alkaline solution and further purified with membrane processing. It was found that the extracts were mainly composed of xylose (50–52%), arabinose (37–39%), galactose (9%) and glucose (1–4%), with an A/X ratio of 0.72–0.77. All the extracts were composed of phenolic compounds, including ferulic acid derivatives such as dimers, trimers and tetramers. The purified extract had a lower concentration of ferulic and p-coumaric acid (0.004 and 0.02 mg/mgdry_weight, respectively) when compared to raw extract (19.30 and 2.74 mg/mgdry_weight, respectively). The same effect was observed for the antioxidant activity, with purified extracts having a lower value (0.17 ± 0.02 µmol TEAC/mg) when compared to the raw extract (2.20 ± 0.35 µmol TEAC/mg). The purified extract showed a greater antiproliferative effect against the HT29 cell line with EC50 = 0.12 ± 0.02 mg/mL when compared to the raw extract (EC50 = 5.60 ± 1.6 mg/mL). Both raw and purified extracts did not show any cytotoxicity to the Caco-2 cell line in the maximum concentration tested (10 mg/mL).10694437engantiproliferative effectarabinoxylanCaco-2 cell linecytotoxicityferulic acidHT29 cell lineAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic Chemistryjournal article10.3390/molecules28155621https://www.scopus.com/pages/publications/85167749418