Teixeira, AlexandraHernández-Rodríguez, Juan F.Wu, LeiOliveira, KevinKant, KrishnaPiairo, PaulinaDiéguez, LorenaAbalde-Cela, Sara2020-10-012020-10-012019-04-012076-3417PURE: 18764626PURE UUID: d37da2d7-76cd-4fd1-b0af-e3564e27f3d1Scopus: 85064069154WOS: 000466547500122http://hdl.handle.net/10362/105031funded by the "Innovative Microfluidic Platform for Analysis of myeloid Leukemia blasts" project (030782). co-funded by FCT and the ERDF through COMPETE2020, the CANCER project (NORTE-01-0145-FEDER-000029) funded through the NORTE-45-2015-02 program.Surface-enhanced Raman scattering (SERS) spectroscopy stands out due to its sensitivity, selectivity, and multiplex ability. The development of ready-to-use, simple, and low-cost SERS substrates is one of the main challenges of the field. In this paper, the intrinsic reproducibility of microfluidics technology was used for the fabrication of self-assembled nanoparticle structures over a paper film. The paper SERS substrates were fabricated by assembling anisotropic particles, gold nanostars (GNSs), and nanorods (NRs) onto paper to offer an extra enhancement to reach ultra-sensitive detection limits. A polydimethylsiloxane PDMS-paper hybrid device was used to control the drying kinetics of the nanoparticles over the paper substrate. This method allowed a high reproducibility and homogeneity of the fabrication of SERS substrates that reach limits of detection down to the picomolar range. This simple and low-cost fabrication of a paper-based sensing device was tested for the discrimination of different cell lineages.5181261engHybridMicrofluidicsNano-biosensorsNanoparticlesNanotechnologySelf-assemblySERSGeneral Materials ScienceInstrumentationGeneral EngineeringProcess Chemistry and TechnologyComputer Science ApplicationsFluid Flow and Transfer Processesjournal article10.3390/app9071387https://www.scopus.com/pages/publications/85064069154