de Miranda, Silvana Spindolade Almeida, Isabela Nevesde Fátima Filardi Oliveira Mansur, Mariade Assis Figueredo, Lida Joucada Silva Carvalho, WâniaHadaad, João Paulo Amaralde Lourdes do Carmo Guimarães Diniz, Jaciaravon Groll, Andreada Silva, Pedro AlmeidaLopes, Maria Luizados Santos, Marcelo CordeiroBrito, Alexandrade Queiroz Mello, Fernanda Carvalhoda Silva Santos Malaquias, ThiagoCroda, JulioPinhata, Juliana Maira Watanabede Oliveira, Rosângela SiqueiraChimara, EricaRossetti, Maria LúciaHalon, Maria LauraLourenço, Maria Cristinade Melo Medeiros, Reginalda FerreiraMontes, Fátima Cristina Onofre FandinhoMachado, DianaViveiros, MiguelKritski, Afrânio Lineu2021-05-012021-05-0120201516-8913PURE: 19727060PURE UUID: 04f7768b-3da4-43d5-8514-9a45c7059216Scopus: 85089808552Scielo: 89132020000100308ORCID: /0000-0001-9676-6251/work/79973341ORCID: /0000-0003-2375-2726/work/79973719http://hdl.handle.net/10362/116609(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.9248046engMDRNitrate reductaseTechnologyTuberculosisBiochemistry, Genetics and Molecular Biology (miscellaneous)Infectious DiseasesMicrobiologySDG 3 - Good Health and Well-beingjournal article10.1590/1678-4324-2020190179https://www.scopus.com/pages/publications/85089808552https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100308&lng=en&nrm=iso&tlng=en