Utilize este identificador para referenciar este registo: http://hdl.handle.net/10362/55746
Título: Probing CHO cells metabolism using metabolomics and fluxomics tools
Autor: Duarte, Tiago Martins da Costa
Orientador: Alves, Paula Marques
Teixeira, Ana Margarida Palma
Palavras-chave: Metabolomics
Data de Defesa: 2016
Resumo: Chinese hamster ovary (CHO) cells are preferred mammalian hosts for industrial production of therapeutic glycoproteins such as monoclonal antibodies (mAbs), used to treat cancer and immunological disorders. In these cells, the glutamine synthetase (GS) expression system has been widely used for efficient selection of high-yielding clones. However, fundamental knowledge on the metabolic behavior of GS-CHO cells in culture and its impact on product yields have not been yet systematized. The overall objective of this thesis was to comprehensively analyse the metabolome and fluxome of CHO cells exploring how the metabolism is affected by clonal variability and culture conditions. This information is then used to generate hypotheses on how metabolic wiring impacts mAb production.(...)
URI: http://hdl.handle.net/10362/55746
Designação: Dissertation presented to obtain the PhD degree in Technological and Engineering Sciences
Aparece nas colecções:ITQB: CBL - PhD Theses

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