ITQB: CPN - MA Dissertations
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- Non-invasive Profile of Embryo Culture Media as a Preimplantation Genetic Diagnosis for AneuploidyPublication . Santos, Cassandra Francelina Sousa; Gonçalves, Luís Gafeira; Nunes, Sofia Gouveia"Aneuploidy is the leading risk factor associated with implantation and embryo’s development failure. Nowadays, the screening methods for early diagnosis of embryo aneuploidy struggle with several limitations such as: invasiveness, be very time-consuming and costly, requirement of highly specialized technicians and several manipulation steps of embryo until diagnosis. For that, the development of a method for detect specific aneuploidy biomarkers in the spent embryo culture media (SECM) is of high interest. The objective of this dissertation was to apply 1H-NMR based metabolomics for the detection of embryo’s ploidy, and, as such, gain more insights in the metabolic alterations among euploid and aneuploid embryos. In this study, the metabolites present in the SECM were quantified using 1H-NMR spectra at two embryo sequential incubation times, and the metabolites concentrations were used to build a PLS-DA classification model.(...)"