Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/23239
Title: Gap geometry dictates epithelial closure efficiency
Author: Ravasio, Andrea
Cheddadi, Ibrahim
Chen, Tianchi
Pereira, Telmo
Ong, Hui Ting
Bertocchi, Cristina
Brugues, Agusti
Jacinto, Antonio
Kabla, Alexandre J.
Toyama, Yusuke
Trepat, Xavier
Gov, Nir
Neves De Almeida, Luís
Ladoux, Benoit
Keywords: COLLECTIVE CELL-MIGRATION
EXTRACELLULAR-MATRIX
DORSAL CLOSURE
WOUND REPAIR
DROSOPHILA EMBRYOS
DYNAMIC-ANALYSIS
GENE-EXPRESSION
STRESS FIBERS
LEADING-EDGE
SINGLE-CELL
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
Issue Date: 9-Jul-2015
Abstract: Closure of wounds and gaps in tissues is fundamental for the correct development and physiology of multicellular organisms and, when misregulated, may lead to inflammation and tumorigenesis. To re-establish tissue integrity, epithelial cells exhibit coordinated motion into the void by active crawling on the substrate and by constricting a supracellular actomyosin cable. Coexistence of these two mechanisms strongly depends on the environment. However, the nature of their coupling remains elusive because of the complexity of the overall process. Here we demonstrate that epithelial gap geometry in both in vitro and in vivo regulates these collective mechanisms. In addition, the mechanical coupling between actomyosin cable contraction and cell crawling acts as a large-scale regulator to control the dynamics of gap closure. Finally, our computational modelling clarifies the respective roles of the two mechanisms during this process, providing a robust and universal mechanism to explain how epithelial tissues restore their integrity.
Peer review: yes
URI: http://www.scopus.com/inward/record.url?scp=84937010465&partnerID=8YFLogxK
http://hdl.handle.net/10362/23239
DOI: http://dx.doi.org/10.1038/ncomms8683
ISSN: 2041-1723
Appears in Collections:Home collection (NMS)

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