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Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/7028

Title: Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254
Authors: López-Cortés, Alejandro
Bursakov, Sergey
Figueiredo, Angelo
Thapper, Anders E.
Todorovic, Smilja
Moura, José J. G.
Ollivier, Bernard
Moura, Isabel
Fauque, Guy
Issue Date: 2005
Publisher: Hindawi Publishing Corporation
Abstract: Two proteins were purified and preliminarily characterized from the soluble extract of cells (310 g, wet weight) of the aminolytic and peptidolytic sulfate-reducing bacterium Desulfovibrio (D.) aminophilus DSM 12254. The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5"-phosphosulfate, APS)reductase, a key enzyme in the microbial dissimilatory sulfate reduction, has been purified in three chromatographic steps(DEAE-Biogel A, Source 15, and Superdex 200 columns). It contains two different subunits with molecular masses of 75 and 18 kDa. The fraction after the last purification step had a purity index (A278 ,m/A388 nm) of 5.34, which was used for further EPR spectroscopic studies. The D. aminophilus APS reductase is very similar to the homologous enzymes isolated from D. gigas and D. desulfuricans ATCC 27774. A tetraheme cytochrome c3 (His-heme iron-His) has been purified in three chromatographic steps (DEAE- Biogel A,Source 15, and Biogel-HTP columns) and preliminarily characterized. It has a purity index ([A.s.s3 nm" A570 nm]rcd/m280nm ox) of 2.9 and a molecular mass of around 15 kDa, and its spectroscopic characterization(NMR and EPR) has been carried out. This hemoprotein presents similarities with the tetraheme cytochrome c3 from Desulfomicrobium (Des.) norvegicurn (NMR spectra, and N-terminal amino acid sequence).
Description: Bioinorganic Chemistry and Applications Volume 3 (2005), Issue 1-2, Pages 81-91
URI: http://hdl.handle.net/10362/7028
ISSN: 1565-3633
Appears in Collections:FCT: DQ - Artigos em revista internacional com arbitragem científica

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