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http://hdl.handle.net/10362/7027
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| Title: | Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 |
| Authors: | Gavel, Olga Yu.
Kladova, Anna V.
Bursakov, Sergey A.
Dias, João M.
Texeira, Susana
Moura, José J. G.
Moura, Isabel
Romão, Maria J.
Trincão, José |
| Issue Date: | 2008 |
| Publisher: | International Union of Crystallography |
| Abstract: | Native zinc/cobalt-containing ATP sulfurylase (ATPS; EC 2.7.7.4; MgATP:
sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was
purified to homogeneity and crystallized. The orthorhombic crystals diffracted
to beyond 2.5 A ° resolution and the X-ray data collected should allow the
determination of the structure of the zinc-bound form of this ATPS. Although
previous biochemical studies of this protein indicated the presence of a homotrimer in solution, a dimer was found in the asymmetric unit. Elucidation of this structure will permit a better understanding of the role of the metal in the activity and stability of this family of enzymes. |
| Description: | Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jul 1;64(Pt 7):593-5 |
| URI: | http://hdl.handle.net/10362/7027 |
| ISSN: | 1744-3091 |
| Appears in Collections: | FCT: DQ - Artigos em revista internacional com arbitragem científica
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