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Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/5842

Título: Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
Autor: Doan, T.
Morlot, C.
Meisner, J.
Serrano, M.
Henriques, A. O.
Moran, C. P.
Rudner, D. Z.
Palavras-chave: BACTERIAL ENDOSPORE FORMATION
SPORULATION MEMBRANE-PROTEIN
TRANSCRIPTION FACTOR
SUBCELLULAR-LOCALIZATION
SIGMA-FACTORS
ENGULFMENT
FORESPORE
CELL
LOCUS
MECHANISM
Issue Date: 1-Jan-2009
Editora: Public Library of Science (PLoS)
Resumo: Sporulation in Bacillus subtilis involves two cells that follow separate but coordinately regulated developmental programs. Late in sporulation, the developing spore (the forespore) resides within a mother cell. The regulation of the forespore transcription factor sigma(G) that acts at this stage has remained enigmatic. sigma(G) activity requires eight mother-cell proteins encoded in the spoIIIA operon and the forespore protein SpoIIQ. Several of the SpoIIIA proteins share similarity with components of specialized secretion systems. One of them resembles a secretion ATPase and we demonstrate that the ATPase motifs are required for sigma(G) activity. We further show that the SpoIIIA proteins and SpoIIQ reside in a multimeric complex that spans the two membranes surrounding the forespore. Finally, we have discovered that these proteins are all required to maintain forespore integrity. In their absence, the forespore develops large invaginations and collapses. Importantly, maintenance of forespore integrity does not require sigma(G). These results support a model in which the SpoIIIA- SpoIIQ proteins form a novel secretion apparatus that allows the mother cell to nurture the forespore, thereby maintaining forespore physiology and sigma(G) activity during spore maturation.
Descrição: Plos Genetics, 5(7): ARTe1000566
URI: http://hdl.handle.net/10362/5842
ISSN: 1553-7390
Appears in Collections:ITQB: MD - Artigos em revista nacional com arbitragem científica

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