DSpace UNL

RUN >
Instituto de Tecnologia Química e Biológica (ITQB) >
ITQB R&D Units >
ITQB: Technology >
ITQB: Engineering Cellular Applications Laboratory - Animal Cell Technology Unit >
ITQB: ECAL - PhD Theses >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/5246

Título: Production optimization of rotavirus-like particles: a system biology approach
Autor: Roldão, António Manuel Missionário
Orientador: Oliveira, Rui M. F.
Carrondo, Manuel J. T.
Issue Date: Jun-2010
Editora: Universidade Nova de Lisboa, Instituto de Tecnologia Química e Biológica
Resumo: Rotavirus-like particles (RLPs), a vaccine candidate against rotavirus disease, were produced by infecting Spodoptera frugiperda Sf-9 cells with genetically engineered recombinant baculoviruses. RLPs are spherically shaped particles composed by three viral proteins (vp) of rotavirus, vp2, vp6 and vp7, arranged in a triple layered structure. A diversity of protein structures, other than the correctly assembled RLP, are observed at the end of a typical production run suggesting that the protein assembly process is rather inefficient. Contaminants such as trimers of vp6 and vp7, vp6 tube-like structures, single-layered vp2 particles, double layered particles of vp2 and vp6 or RLPs lacking one or more subunits represent almost 88% of the total mass of proteins expressed. Thus, optimal control of protein expression concomitant with efficient particle assembly are critical factors for economical RLP production in the baculovirus/insect cells system.
Descrição: Dissertation presented to obtain a Ph.D. degree in Engineering and Technology Sciences, Systems Biology at the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa
URI: http://hdl.handle.net/10362/5246
Appears in Collections:ITQB: ECAL - PhD Theses

Files in This Item:

File Description SizeFormat
Roldao_2010.pdf9,99 MBAdobe PDFView/Open
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Universidade Nova de Lisboa  - Feedback
Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE