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Title: Reproducing stone monument photosynthetic-based colonization under laboratory conditions
Authors: Macedo, Maria Filomena
Miller, Ana Zélia
Laiz, Leonila
Miguel Gonzalez, Juan
Dionísio, Amélia
Keywords: Biodeterioration
Laboratory chamber
Phototrophic microorganisms
Colonization experiment
Issue Date: Nov-2008
Publisher: Elsevier Ltd.
Abstract: In order to understand the biodeterioration process occurring on stone monuments, we analyzed the microbial communities involved in these processes and studied their ability to colonize stones under controlled laboratory experiments. In this study, a natural green biofilm from a limestone monument was cultivated, inoculated on stone probes of the same lithotype and incubated in a laboratory chamber. This incubation system, which exposes stone samples to intermittently sprinkling water, allowed the development of photosynthetic biofilms similar to those occurring on stone monuments. Denaturing gradient gel electrophoresis (DGGE) analysis was used to evaluate the major microbial components of the laboratory biofilms. Cyanobacteria, green microalgae, bacteria and fungi were identified by DNA-based molecular analysis targeting the 16S and 18S ribosomal RNA genes. The natural green biofilm was mainly composed by the Chlorophyta Chlorella, Stichococcus, and Trebouxia, and by Cyanobacteria belonging to the genera Leptolyngbya and Pleurocapsa. A number of bacteria belonging to Alphaproteobacteria, Bacteroidetes and Verrucomicrobia were identified, as well as fungi from the Ascomycota. The laboratory colonization experiment on stone probes showed a colonization pattern similar to that occurring on stone monuments. The methodology described in this paper allowed to reproduce a colonization equivalent to the natural biodeteriorating process.
Description: Science of the total environment 405(2008) 278-285
ISSN: 0048-9697
Appears in Collections:FCT: DCR - Artigos em revista internacional com arbitragem cientifica

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