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Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/4199

Title: A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
Authors: Sá-Nogueira, Isabel de
Ferreira, Mário José
Issue Date: 2010
Publisher: American Society for Microbiology
Citation: Ferreira M.J. and I. de Sá-Nogueira (2010) A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis. Journal of Bacteriology 192 (20): 5312-5318
Abstract: Bacillus subtilis is able to utilize arabinopolysaccharides derived from plant biomass. Here, by combining genetic and physiological analyses we characterize the AraNPQ importer and identify primary and secondary transporters of B. subtilis involved in the uptake of arabinosaccharides. We show that the ABC-type importer AraNPQ is involved in the uptake of α-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units. Although this system is the key transporter for α-1,5-arabinotriose and α-1,5-arabinotetraose, the results indicate that α-1,5-arabinobiose also is translocated by the secondary transporter AraE. This broad-specificity proton symporter is the major transporter for arabinose and also is accountable for the uptake of xylose and galactose. In addition, MsmX is shown to be the ATPase that energizes the incomplete AraNPQ importer. Furthermore, the results suggest the existence of at least one more unidentified MsmX-dependent ABC importer responsible for the uptake of nonlinear α-1,2- and α-1,3-arabinooligosaccharides. This study assigns MsmX as a multipurpose B. subtilis ATPase required to energize different saccharide transporters, the arabinooligosaccharide-specific AraNPQ-MsmX system, a putative MsmX-dependent ABC transporter specific for nonlinear arabinooligosaccharides, and the previously characterized maltodextrin-specific MdxEFG-MsmX system.
Description: Journal of Bacteriology (Out 2010) 5312-5318
URI: http://hdl.handle.net/10362/4199
ISSN: 0021-9193 (Print)
1098-5530 (Online)
Appears in Collections:FCT: DCV - Artigos em revista internacional com arbitragem científica

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