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Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/1678

Title: Hydrogen evolution and consumption in AOT–isooctane reverse micelles by Desulfovibrio gigas hydrogenase
Authors: Moura, José J. G.
Andrade, Susana L. A.
Keywords: Hydrogenase
Desulfovibrio gigas
Reverse micelles
Hydrogen production and consumption
Issue Date: 2002
Publisher: Elsevier Science Inc.
Abstract: The enzyme hydrogenase isolated from the sulphate reducing anaerobic bacterium Desulfovibrio gigas was encapsulated in reverse micelles of AOT–water–isooctane. The enzyme ability to consume molecular hydrogen was studied as a function of the micelle size (given by Wo = [H2O]/[organic solvent]). A peak of catalytic activity was obtained for Wo = 18, a micelle size theoretically fitting the heterodimeric hydrogenase molecule. At this Wo value, the recorded catalytic activity was slightly higher than in a buffer system(Kcat = 169.43 s−1 against the buffer value of 151 s−1). The optimal buffer used to encapsulate the enzyme was found to be imidazole 50 mM, pH 9.0. The molecular hydrogen production activity was also tested in this reverse micelle medium.
URI: http://hdl.handle.net/10362/1678
ISSN: 0141-0229
Other Identifiers: Enzyme and Microbial Technology 31(2002)398–402
Appears in Collections:FCT: DQ - Artigos em revista internacional com arbitragem científica

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