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Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/1677

Título: Bilirubin directly disrupts membrane lipid polarity and fluidity, protein order, and redox status in rat mitochondria
Autor: Moura, José J. G.
Rodrigues, Cecília M. P.
Solá, Susana
Brito, Maria A.
Brites, Dora
Palavras-chave: Bilirubin cytotoxicity
Cytochrome c
Electron paramagnetic resonance spectroscopy
Mitochondrial membrane structure
Issue Date: 2002
Editora: Elsevier Science B.V.
Resumo: Background/Aims: Unconjugated bilirubin (UCB) impairs crucial aspects of cell function and induces apoptosis in primary cultured neurones. While mechanisms of cytotoxicity begin to unfold, mitochondria appear as potential primary targets. Methods: We used electron paramagnetic resonance spectroscopy analysis of isolated rat mitochondria to test the hypothesis that UCB physically interacts with mitochondria to induce structural membrane perturbation, leading to increased permeability, and subsequent release of apoptotic factors. Results: Our data demonstrate profound changes on mitochondrial membrane properties during incubation with UCB, including modified membrane lipid polarity and fluidity (P , 0:01), as well as disrupted protein mobility(P , 0:001). Consistent with increased permeability, cytochrome c was released from the intermembrane space(P , 0:01), perhaps uncoupling the respiratory chain and further increasing oxidative stress (P , 0:01). Both ursodeoxycholate, a mitochondrial-membrane stabilising agent, and cyclosporine A, an inhibitor of the permeability transition, almost completely abrogated UCB-induced perturbation. Conclusions: UCB directly interacts with mitochondria influencing membrane lipid and protein properties, redox status, and cytochrome c content. Thus, apoptosis induced by UCB may be mediated, at least in part, by physical perturbation of the mitochondrial membrane. These novel findings should ultimately prove useful to our evolving understanding of UCB cytotoxicity.
URI: http://hdl.handle.net/10362/1677
ISSN: 0168-8278
Outros identificadores: Journal of Hepatology 36(2002)335–341
Appears in Collections:FCT: DQ - Artigos em revista internacional com arbitragem científica

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