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Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/1667

Título: Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
Autor: Moura, José J. G.
Simes, Dina C.
Bebianno, Maria João
Palavras-chave: Metallothionein
Clam
Ruditapes decussatus
Cd
Toxicity
Issue Date: 2003
Editora: Elsevier
Resumo: Metallothioneins (MT) were obtained after purification from metal-exposed clams (Ruditapes decussatus) using gelpermeation and ion-exchange chromatography. Four cadmium /metallothioneins (CdMTs) were resolved by ionexchange chromatography and they all had similar molecular weights, high cadmium content and an absorption spectra indicative of the presence of characteristic Cd /S aggregates. The NH2-terminal sequence suggests the presence of atleast two class I clam MT isoforms. For the other two putative clam CdMTs isolated, the results of the amino acid determination were inconclusive. One was slightly contaminated and the other one had a blocked NH2-terminal. These clam metalothioneins contain glycine, which seems to be a common feature of molluscan MT family and exhibited more similarity to oysters than to mussels. Further investigation on the inducibility of these isoforms will be necessary if clams are to be used as biomarkers of metal exposure.
Descrição: Aquatic Toxicology 63 (2003) 307-318
URI: http://hdl.handle.net/10362/1667
ISSN: 0166-445X
Appears in Collections:FCT: DQ - Artigos em revista internacional com arbitragem científica

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