http://hdl.handle.net/10362/3563 2013-05-26T05:04:55Z 2013-05-26T05:04:55Z Miguel, Ana Sofia http://hdl.handle.net/10362/8594 2013-01-22T17:34:31Z 2012-09-01T00:00:00Z

Title: Quantum dots: synthesis, functionalization and bioconjugation for biological applications Authors: Miguel, Ana Sofia Abstract: The main goal of this Doctoral work was to develop a fluorescent biomarker to identify antigenic proteins associated with specific parasites. Although it is known that fluorescent techniques making use of standard organic dyes are widely used for this purpose, the development of a new method was proposed using nanotechnology; in particular the use of nano optical reporters also called quantum dots (QDs). In order to achieve this goal, the research work was divided into four main tasks: (1) synthesis and characterization of CdSe/ZnS core-shell QDs; (2) design of dihydrolipoic acid (DHLA) ligands appended to oligo and poly (ethylene) glycols (PEG) with different functional groups to generate biocompatible QDs; (3) bioconjugation of QDs to monoclonal antibodies based on sophisticated protocols and (4) some chemical and biological applications of the synthesized non-conjugated and conjugated nanoparticles.(...) Description: Dissertation presented to obtain the Ph.D degree in Engineering Sciences and Technology

2012-09-01T00:00:00Z